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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant purpose in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two extra splice variants had been described but specific nucleotide sequence information just isn't but out there.
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Blocking peptides are peptides that bind particularly to your goal antibody and block antibody binding. These peptide commonly consists of the epitope acknowledged via the antibody.
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This guideline outlines further more feasible optimization of mobile and immunoassay parameters to be certain the absolute best outcomes are...
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This manual outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no longer bind to the epitope around the target protein. By evaluating the staining from the blocked antibody as opposed to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to the hugely conserved cyclin household, whose customers are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells development by way of S phase.
The measurement of protein get more info phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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